Analysis Note

ControlMacrophages, monocytes, lymphoid organs.

Application

Immunohistochemistry:
Frozen sections and Formalin fixed, paraffin embedded tissue sections (requires trypsin or pronase pre-treatment).

Western Blotting:
A previous lot of this antibody was used in western blot.

Flow cytometry:
1:100-1:200 of a previous lot was used. (Use 10 µL of working dilution for 10^6 cells in 100 µL).

Optimal working dilutions must be determined by the end user.

This Anti-Macrophages/Granulocytes Antibody, clone OX-41 is validated for use in FC, IC, IH, IH(P), WB for the detection of Macrophages/Granulocytes.

Research CategoryInflammation & Immunology

Research Sub CategoryInflammation & Autoimmune Mechanisms

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Macrophages and granulocytes express Signal regulatory protein (SIRP), which is a transmembrane glycoprotein involved in the down regulation of tyrosine kinase receptor signal transduction induced by cell adhesion, growth factors or insulin. They are immunoglobulin-like cell surface receptor for CD47. By CD47 binding there is a negative regulation of phagocytosis, mast cell activation and dendritic cell activation that prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Immunogen

Rat peritoneal macrophages.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB1407

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Purified mouse monoclonal IgG2a in buffer containing 0.09% sodium azide.

Quality

Routinely evaluated by Immunocytochemistry on Macrophages/Granulocytes.

Immunocytochemistry Analysis:
Confolcal fluorescent analysis of Macrophages/ Granulocytes using anti-Macrophages/ Granulocytes mouse monoclonal antibody.

Specificity

MAB1407P recognizes rat SIRP (Signal regulatory protein), which is expressed selectively by myeloid cells and by neurons. This monoclonal binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a and CD47.
Labels most macrophages including resident peritoneal, alveolar and activated macrophages. Recognition of other macrophages is limited to only occasional kupffer cells. In addition it labels granulocytes and a population of dendritic cells. It also labels brain extensively.

Expected to cross-react with rat and mouse.

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Target description

56 kDa